Tag: monoclonal antibodies

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Multiple choice biology immunity monoclonal antibodies antibody production and vaccination biotechnology

In hybridoma technology.

  1. B-cells are fused with myeloma cells

  2. T-cells are fused with myeloma cells

  3. B-cells are fused with T-cells

  4. None of the above
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

In hybridoma technology antibody producing B cells are fused with myeloma cells. These hybrid cells therefore acquire the capability to generate antibodies and also have the ability to live on indefinitely as it is a hybrid of myeloma cell.  Hence, they are able to generate a large number of monoclonal antibodies. 

So, the correct answer is 'B-cells are fused with myeloma cells'

Multiple choice biology immunity monoclonal antibodies antibody production and vaccination biotechnology

Which is employed for synthesis of monoclonal antibody by hybridoma technique?

  1. RBC

  2. Liver cells

  3. Tumour cells

  4. Nerve cells
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

The hybridoma technique involves fusing an antibody-producing B-cell with a myeloma (cancerous tumour) cell. The tumour cell provides the property of immortality, allowing the hybridoma to divide indefinitely and produce monoclonal antibodies.

Multiple choice biology immunity monoclonal antibodies antibody production and vaccination biotechnology

The hybridoma cells are 

  1. Cells of oncogenes and brain

  2. Hybrid activated B lymphocytes fused with myeloma cells

  3. Hybrid cells of oncogenes fused with myeloma cells

  4. Transformed cells
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

A hybridoma cell is produced in the laboratory from the fusion of an antibody-producing lymphocyte and a nonantibody-producing cancer cell, usually a myeloma or lymphoma. It proliferates and produces a continuous supply of a specific monoclonal antibody.

Therefore, the correct answer is option B.

Multiple choice biology immunity monoclonal antibodies antibody production and vaccination biotechnology

Kohler and Milstein developed the Hybridoma technology for the production of

  1. Myelomas

  2. Steroid conversion

  3. Monoclonal antibodies

  4. Immobilised enzymes
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Kohler and Milstein created hybridomas by fusing malignant myeloma cells with antibody-producing B cells. The myeloma cells have the ability to replicate indefinitely and are clonogenic they will form clones when grown in vitro while the antibody producing b-cells will release the specific antibody. So, the correct answer is option C.

Multiple choice biology immunity monoclonal antibodies antibody production and vaccination biotechnology

Antibodies made outside the body by hybrid cell cultures are known as

  1. Biotechnological antibodies

  2. Specific antibodies

  3. Hybrid antibodies

  4. Monoclonal antibodies
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Monoclonal antibodies are antibodies that are made by identical immune cells that are all clones of a unique parent cell. Monoclonal antibodies can have monovalent affinity, in that they bind to the same epitope (the part of an antigen that is recognized by the antibody). In contrast, polyclonal antibodies bind to multiple epitopes and are usually made by several different plasma cell (antibody secreting immune cell) lineages. Bispecific monoclonal antibodies can also be engineered, by increasing the therapeutic targets of one single monoclonal antibody to two epitopes. Given almost any substance, it is possible to produce monoclonal antibodies that specifically bind to that substance; they can then serve to detect or purify that substance. This has become an important tool in biochemistry, molecular biology, and medicine. 

So the correct option is 'monoclonal antibodies'.

Multiple choice biology immunity monoclonal antibodies antibody production and vaccination biotechnology

Choose the correct answers from the alternatives given.
Monoclonal antibodies are

  1. produced by clones formed from memory cells

  2. used to produce large quantities of interferon

  3. produced by cultures of hybridoma cells

  4. produced by clones of 1-cells fused with tumor cells
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Monoclonal antibodies are the antibodies that are made by identical immune cells that are all clones of a unique parent cell. In contrast, polyclonal antibodies bind to multiple epitopes and are usually made by several different plasma cells.

So, the correct option is 'Produced by cultures of hybridoma cells'.