Tag: bio-chemistry

Questions Related to bio-chemistry

After the biosynthetic phase, the product is separated and purified by the process called as:

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Agarose gel electrophoresis

  2. PCR

  3. Downstream processing

  4. Insertional inactivation

Show answer
Correct Option: C
Explanation:
After the biasynthetic, the product is seprated and parified by the process called doren stream processing. It involves $5$ stages.
$(1)$ solid- liquid sepretion
$(2)$ Retrase of intractual products
$(3)$ concentration
$(4)$ Purification by chronatography
$(5)$ Formulation
So, the correct answer is $[C]$ Downstrain processing.

What must be done before placing DNA into the electrophoretic chamber?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. It must be ground up with mortar and pestle

  2. It must be cut by restricition endonucleases

  3. It must be treated with RNAase

  4. None of these

Show answer
Correct Option: B
Explanation:

Answer is option B i.e. "It must be cut by restriction endonucleases"
Electrophoresis is a method for separation of macro-molecules like DNA. Preparing the DNA for electrophoresis is an important step. DNA is "digested" or cut by restriction enzymes before being run on a gel so that DNA can be separated based on DNA size, fragment and length.

Purified DNA ultimately precipitate out after the addition of chilled ethanol. This DNA that separate out can be removed by _____________.

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Electrophoresis

  2. Downstream processing

  3. PCR

  4. Spooling

Show answer
Correct Option: D
Explanation:

Answer is option D i.e. "Spooling"
At the last step of DNA extraction, purified DNA precipitates out after the addition of chilled ethanol. This DNA appears as collection of fine threads in the suspension which can be spool over the glass rod, this method is called as DNA spooling.

Agarose which is extracted from sea weeds is most widely used in

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Gel electrophoresis

  2. Spectrophotometry

  3. Tissue culture

  4. PCR

Show answer
Correct Option: A
Explanation:

Agarose is the supporting structure in the cell walls of certain species of algae and which is released on boiling. Agarose is a polysaccharide polymer extracted from seaweed. Slabs of agarose gels are used for electrophoresis.
Thus, the correct answer is option A.

The technique used for separating molecules by passing electric current is

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Fractionation

  2. Centrifugation

  3. Electrophoresis

  4. ELISA

  5. Chromatography

Show answer
Correct Option: C
Explanation:
Electrophoresis is a technique, in which particles of different sizes and charges are separated due to their movement to different distances under the influence of an electric field, e.g : nucleic acid, proteins, amino acids, nucleotides etc.
Chromatography is a technique of separating molecular components of a mixture by using the principle of partition coefficient.
Cell fractionation is the technique of separating and rupturing cells and separating the cellular components for study. It has two steps - homogenisation (rupturing of cells) and differential centrifugation (separation of different components of homogenate). 
ELISA stands for Enzyme Linked immunosorbent Assay. It is an assay procedure used in diagnosis of AIDS.

A method which uses paper or film with different electric poles at the two ends and is used for separation of charged particles is called

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Electrolysis

  2. Electrophoresis

  3. Electroplating

  4. Thin layer chromatography

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Correct Option: B

Agarose gel used to separate

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Carbohydrates.

  2. Fats.

  3. Proteins.

  4. Both A and B.

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Correct Option: C

Electric field is used in separating chemicals in which method?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Ion exchange chromatography

  2. Electrophoresis

  3. Thin layer chromatography

  4. Column chromatography

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Correct Option: B

Electrophoresis is employed for

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Separating cell components.

  2. Separating charged particles.

  3. Reverse osmosis.

  4. In vitro storage of cell components.

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Correct Option: A

The base material that can be used in electrophoresis is

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Polyacrylamide.

  2. Agarose.

  3. Carrageenan.

  4. Both A and B.

Show answer
Correct Option: D