Biology

Molecular Biology and Genetics

717 Questions

Molecular biology and genetics explore the structure and function of DNA, RNA, genetic mutations, and heredity. Understanding these building blocks of life is essential for most biology examinations. Review these practice questions to test your molecular genetics knowledge.

DNA structure and basesGenetic mutationsNucleic acids compositionDNA sequencing and replication

Molecular Biology and Genetics Questions

Multiple choice
  1. P - 1; Q - 2; R - 3; S - 4

  2. P - 1; Q - 3; R - 2; S - 4

  3. P - 2; Q - 1; R - 4; S - 3

  4. P - 4; Q - 1; R - 2; S - 3

  5. P - 3; Q - 2; R - 4; S - 1

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Adenoviridae is a family of non-enveloped double-stranded DNA viruses. Caliciviridae is a family of enveloped + strand single-stranded RNA viruses. Picornaviridae is a family of non-enveloped + strand single-stranded RNA viruses. Poxviridae is a family of enveloped double-stranded DNA viruses.

Multiple choice
  1. They are beaded structures, each with a histone core wrapped in a left-handed super helix of DNA.

  2. The beads are linked by a DNA chain.

  3. Each contains two molecules of histones H2A, H2B, H3 and H4.

  4. The primary structure of the histones is a characteristic of the cell type and the species.

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Histone proteins (H2A, H2B, H3, H4) are highly conserved across species and cell types. Their primary amino acid sequence is remarkably similar from yeast to humans, not characteristic of specific cell types.

Multiple choice
  1. P and Q

  2. Q and R

  3. P, Q and R

  4. P, Q, R and S

Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Virusoids are satellite nucleic acids that depend on helper viruses for replication. They are indeed non-coding molecules and some exhibit ribozyme activity (catalytic RNA). However, they do not have a capsid - that would distinguish them from viroids, which are also naked infectious RNA molecules.

Multiple choice
  1. the “other strand” of single-stranded DNA

  2. in no need of template for its synthesis

  3. a base sequence of the vector DNA

  4. produced by reverse transcription

  5. produced by transcription.

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Complementary DNA (cDNA) is produced by reverse transcription.

Multiple choice
  1. Only (a)

  2. Only (b)

  3. Only (c)

  4. All of the above

  5. None of the above

Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Statement (a) is incorrect. Genes are found on chromosomes at fixed, not variable positions.

Multiple choice

Which of these can be compared to the human genome after the new finding?

Directions: Answer the given question based on the following passage:

Junk. Barren. Non-functioning. Dark matter. That’s how scientists had described the 98% of human genome that lies between our 21,000 genes, ever since our DNA was first sequenced about a decade ago. The disappointment in those descriptors was intentional and palpable.
It had been believed that the human genome — the underpinnings of the blueprint for the talking, empire-building, socially evolved species that we are — would be stuffed with sophisticated genes, coding for critical proteins of unparalleled complexity. But when all was said and done, and the Human Genome Project finally determined the entire sequence of our DNA in 2001, researchers found that the 3 billion base pairs that comprised our mere 21,000 genes made up a paltry 2% of the entire genome. The rest, geneticists acknowledged with unconcealed embarrassment, was an apparent biological wasteland.
But it turns out they were wrong. In an impressive series of more than 30 papers published in several journals, including Nature, Genome Research, Genome Biology, Science and Cell, scientists now report that these vast stretches of seeming “junk” DNA are actually the seat of crucial gene-controlling activity — changes that contribute to hundreds of common diseases. The new data come from the Encyclopaedia of DNA Elements project, or ENCODE, a $123 million endeavor begun by the National Human Genome Research Institute (NHGRI) in 2003, which includes 442 scientists in 32 labs around the world.
ENCODE has revealed that some 80% of the human genome is biochemically active. “What is remarkable is how much of the genome is doing at least something. It has changed my perception of the genome,” says Ewan Birney, ENCODE’s lead analysis coordinator from the European Bioinformatics Institute.
Rather than being inert, the portions of DNA that do not code for genes contain about 4 million so-called gene switches, transcription factors that control when our genes turn on and off and how much protein they make, not only affecting all the cells and organs in our body, but doing so at different points in our lifetime. Somewhere amidst that 80% of DNA, for example, lie the instructions that coax an uncommitted cell in a growing embryo to form a brain neuron, or direct a cell in the pancreas to churn out insulin after a meal, or guide a skin cell to bud off and replace a predecessor that has sloughed off.
“What we learned from ENCODE is how complicated the human genome is, and the incredible choreography that is going on with the immense number of switches that are choreographing how genes are used,” Eric Green, director of NHGRI, told reporters during a teleconference discussing the findings. “We are starting to answer fundamental questions like what are the working parts of the human genome, parts list of the human genome and what those parts do.”

 

  1. The director of a play who dictates what roles the various actors should play and how they should play it.

  2. The top management of a company dictating what the workers will do.

  3. Each member of an ant colony passing messages to each other and coordinating to do the work.

  4. Majority of students of the class assigned some leadership activity related to a festival.

  5. The chain of command in an army unit where instructions flow from top to bottom.

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

According to the new finding, most part of the genome is doing at least some activity or the other. Similarly a number of students are doing some activity related to the food festival. Most of the students are doing some activity related to the food festival. This answer choice is correct. Majority of students are performing leadership activities (controlling activities just like the DNA in the human genome).

Multiple choice

All of the following are true according to the passage except

Directions: Answer the given question based on the following passage:

Junk. Barren. Non-functioning. Dark matter. That’s how scientists had described the 98% of human genome that lies between our 21,000 genes, ever since our DNA was first sequenced about a decade ago. The disappointment in those descriptors was intentional and palpable.
It had been believed that the human genome — the underpinnings of the blueprint for the talking, empire-building, socially evolved species that we are — would be stuffed with sophisticated genes, coding for critical proteins of unparalleled complexity. But when all was said and done, and the Human Genome Project finally determined the entire sequence of our DNA in 2001, researchers found that the 3 billion base pairs that comprised our mere 21,000 genes made up a paltry 2% of the entire genome. The rest, geneticists acknowledged with unconcealed embarrassment, was an apparent biological wasteland.
But it turns out they were wrong. In an impressive series of more than 30 papers published in several journals, including Nature, Genome Research, Genome Biology, Science and Cell, scientists now report that these vast stretches of seeming “junk” DNA are actually the seat of crucial gene-controlling activity — changes that contribute to hundreds of common diseases. The new data come from the Encyclopaedia of DNA Elements project, or ENCODE, a $123 million endeavor begun by the National Human Genome Research Institute (NHGRI) in 2003, which includes 442 scientists in 32 labs around the world.
ENCODE has revealed that some 80% of the human genome is biochemically active. “What is remarkable is how much of the genome is doing at least something. It has changed my perception of the genome,” says Ewan Birney, ENCODE’s lead analysis coordinator from the European Bioinformatics Institute.
Rather than being inert, the portions of DNA that do not code for genes contain about 4 million so-called gene switches, transcription factors that control when our genes turn on and off and how much protein they make, not only affecting all the cells and organs in our body, but doing so at different points in our lifetime. Somewhere amidst that 80% of DNA, for example, lie the instructions that coax an uncommitted cell in a growing embryo to form a brain neuron, or direct a cell in the pancreas to churn out insulin after a meal, or guide a skin cell to bud off and replace a predecessor that has sloughed off.
“What we learned from ENCODE is how complicated the human genome is, and the incredible choreography that is going on with the immense number of switches that are choreographing how genes are used,” Eric Green, director of NHGRI, told reporters during a teleconference discussing the findings. “We are starting to answer fundamental questions like what are the working parts of the human genome, parts list of the human genome and what those parts do.”

 

  1. Ewan Bimey had a different idea about the human genome before.

  2. When the DNA was first sequenced, there was a certain degree of disappointment.

  3. What was once thought of as waste controls many bodily functions rhythmically.

  4. A large number of researchers and scientific labs contributed to the ENCODE project.

  5. Most of our genes are involved in some activity or other.

Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

We need to look for an answer choice that does not completely agree with the contents of the passage. The passage does say that what was once thought to be junk DNA actually controls many bodily functions but the passage does not say that it controls these bodily functions rhythmically. This answer choice is correct. The passage says that the junk DNA is the seat of gene controlling activity but does not say that these DNA do their activity rhythmically.

Multiple choice
  1. They invariably contain DNA.

  2. They multiply only in the host cells.

  3. They occur only inside bacteria.

  4. They are bigger than bacteria.

  5. Their genetic material is RNA not DNA.

Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

This is true as they multiply only in the host cells.

Multiple choice
  1. Both heterochromatin and euchromatin

  2. Only heterochromatin

  3. Neither euchromatin or heterochromatin but the nucleolus

  4. Only euchromatin

Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

As replication of DNA occurs in S-phase of cell cycle, DNA of both euchromatin and heterochromatin will undergo replication process and they take radioactive adenosine from the medium for the formation of complementary base pairs and hence, both chromatin will show Radioactive Adenosine. Heterochromatin is densely packed and deeply stained chromatin present in the periphery of nucleus responsible for regulation of gene expression due to position effect variegation. Euchromatin is loosely packed and lightly stained chromatin present in nucleus which contains almost 92% of human genome and undergoes transcription.

Multiple choice
  1. are double stranded

  2. have similar sugars

  3. are polymers of nucleotides

  4. have similar pyrimidine

Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Both DNA and RNA are nucleic acids composed of nucleotide monomers, which is their fundamental similarity. The key differences are that DNA contains deoxyribose sugar and is typically double-stranded, while RNA contains ribose sugar and is usually single-stranded. DNA uses thymine as a pyrimidine base, whereas RNA uses uracil.

Multiple choice
  1. Adenine

  2. Guanine

  3. 5-bromouracil

  4. 5-methylcytocine

Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

5-Bromouracil (or 5-bromo-2,4(1H,3H)-pyrimidinedione or 5-BrU or 5-BU) is a brominated derivative of uracil that acts as an antimetabolite or base analog, substituting for thymine in DNA, and can induce DNA mutation