Multiple choice Once a peptide bond is formed, what happens before the second last tRNA can detach? The ribosome moves along the mRNA by one codon Another tRNA attaches to the mRNA with its anticodon The ribosome detaches The polypeptide chain detaches Reveal answer Fill a bubble to check yourself A Correct answer Explanation After a peptide bond is formed between the amino acids, translocation occurs, meaning the ribosome moves along the mRNA by one codon. This movement shifts the now-empty tRNA into the exit (E) site, allowing it to detach and leave the ribosome.