Multiple choice

What is the last step for gene transfer?

  1. Digestion of gene and vector (by restriction endonuclease)

  2. Isolation of gene and vector (by PCR)

  3. Selection and expression of transgenic construct

  4. Ligation of gene and vector (by DNA ligase)
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C Correct answer
Explanation

In gene transfer, the final step is the selection and expression of the transgenic construct to ensure the host organism has successfully incorporated and is expressing the desired gene.

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