Multiple choice A photoautotrophic micropropagation system can be established by increasing the sucrose concentration in the culture medium CO2 concentration in the culture medium agar concentration in the culture medium NH4+ concentration in the culture medium Reveal answer Fill a bubble to check yourself B Correct answer Explanation In a photoautotrophic micropropagation system, plantlets are grown without sugar in the medium and rely on photosynthesis, requiring an enhanced supply of carbon dioxide in the culture vessel to support autotrophic growth.