Multiple choice

Horseradish peroxidase and alkaline phosphatase are the two enzymes commonly utilized as reagents in ELISA, because these enzymes

  1. are colored proteins

  2. are very small

  3. have high turnover number

  4. bind to ELISA plates
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

In ELISA assays, enzymes like horseradish peroxidase and alkaline phosphatase are chosen because they possess very high turnover numbers, meaning they rapidly convert a large amount of substrate into a detectable colored or fluorescent product, thus providing high assay sensitivity.

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