Which of the following vectors has been designed to clone cDNA?
Lambda GT10 is a genetically engineered version of the wild type lambda virus. It has been changed in two important ways in order to make it more useful for molecular biological manipulations. First, a large piece of unnecessary DNA (the stuffer fragment) was removed. This creates room in the genome to clone a piece of foreign DNA (an insert) into the virus, which will then be replicated along with the viral genome. This means that we can use lambda GT10 to amplify a cloned piece of DNA and make many copies of it. Second, lambda GT10 has been modified to only use the lytic cycle when it is grown in an appropriate host. For this reason, when you grow the phage on a lawn of the appropriate strain of E. coli, the phage will lyse the bacteria and you will see a clear spot on the cloudy film of E. coli