Multiple choice

Which of the following vectors is used for obtaining single stranded copies of cloned DNA?

  1. pUC7

  2. pSC101

  3. phage M13 vector

  4. phasmid vectors

  5. shuttle vectors
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C Correct answer
Explanation

They are used for obtaining single stranded copies of cloned DNA, which are especially suited for DNA sequencing. They are derived from 6.4kb genome of E.coli filamentous bacteriophage M13. This phage has single-stranded DNA genome in phage particle that converts into double-stranded intermediate within host cell.

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