Multiple choice

A single nucleotide polymorphism is a variation, at a single position in a DNA sequence among individuals. Which of the following techniques of epigenetics is used to find the single nucleotide polymorphism?

  1. Direct sequencing

  2. Pyrosequencing

  3. Methylation-sensitive single-strand conformation analysis (MS-SSCA)

  4. High resolution melting analysis (HRM)

  5. Methylation-sensitive single-nucleotide primer extension (MS-SnuPE)
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D Correct answer
Explanation

High-resolution melting analysis (HRM) is used to find the single nucleotide polymorphism. The method is based on the technique of real time PCR. The extent of DNA methyaltion is calculated from the C : T ratio as determined by changes in temperature and subsequent release of the fluorescent dye.

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