Multiple choice Which of the following enzymes is required for nitrogen fixation? Nitrogen deaminase Nitrodioxidase Amino acid decarboxylase Nitrogenase Reveal answer Fill a bubble to check yourself D Correct answer Explanation The enzyme nitrogenase is required for nitrogen fixation.All nitrogenases have an iron- and sulfur-containing cofactor that includes a heterometal complex in the active site (e.g., FeMoCo).