Multiple choice In the tissues, high concentration of carbon dioxide increases the affinity of haemoglobin to both oxygen and hydrogen increases the affinity of haemoglobin to oxygen but decreases its affinity to hydrogen decreases the affinity of haemoglobin to oxygen but increases its affinity to hydrogen decreases the affinity of haemoglobin to both oxygen and hydrogen Reveal answer Fill a bubble to check yourself C Correct answer Explanation The Bohr effect states that high CO2 concentrations increase hydrogen ion concentration (lower pH), which decreases hemoglobin's affinity for oxygen, facilitating oxygen release in tissues.