Multiple choice general knowledge science & technology The nucleotide sequence of the first tRNA was determined in 1965 1968 1985 1999 Reveal answer Fill a bubble to check yourself A Correct answer Explanation The first complete nucleotide sequence of a tRNA molecule was determined in 1965 by Robert W. Holley and colleagues at Cornell University, for alanine tRNA from yeast. This was a landmark achievement in molecular biology.