Multiple choice

Which of the following is a fluorescent nuclear stain that is excited by ultraviolet light, showing blue fluorescence when bound to DNA?

  1. Carmine

  2. DAPI

  3. Coomassie blue

  4. IKI

  5. Hoechst stains
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

DAPI is a fluorescent nuclear stain, excited by ultraviolet light and showing strong blue fluorescence when bound to DNA . DAPI binds with A=T rich repeats of chromosomes. DAPI is also not visible with regular transmission microscopy. It may be used in living or fixed cells. DAPI-stained cells are especially appropriate for cell counting.

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