Multiple choice

Acridine orange is used as a stain for

  1. DNA and RNA

  2. proteins

  3. carbohydrate

  4. lipids

Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Acridine orange is a metachromatic fluorescent dye that intercalates into nucleic acids. It binds to DNA emitting green fluorescence and to RNA emitting orange-red fluorescence when viewed under fluorescence microscope. This differential staining allows visualization of both DNA and RNA in cells.