Multiple choice

Which of the following staining is used in the disease Diphtheria to demonstrate the presence or absence of metachromatic granules?

  1. Gram staining

  2. Negative staining

  3. Albert’s staining

  4. Both Gram staining and Albert’s staining

  5. Silver staining
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D Correct answer
Explanation

Both Gram staining and Albert’s staining are used for C.dihtheria demonstration. In all cases of suspected cases of Diphtheria, stain one of the smears with Gram stain. If the Gram stained smear shows morphology suggestive of C.diptheria, proceed to do Albert staining which demonstrates the presence or absence of metachromatic granules. These granules appear bluish black whereas the body of bacilli appear green or bluish green.

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