Multiple choice Net gain of ATP in prokaryotes when a molecule of glucose is oxidized is: 40 38 36 34 20 Reveal answer Fill a bubble to check yourself B Correct answer Explanation A total of 38 ATP are produced when one glucose molecule is oxidised. Glycolysis gives 8 ATP. One pyruvate on entering TCA gives 15 ATP. As two pyruvate are formed in glycolysis, 30 ATP will be produced from these two. This gives a total of 38 ATP.