Lysosomal Storage Disorders are characterized by deficiency of specific lysosomal hydrolase enzymes, leading to accumulation of substrates within lysosomes. The primary defect is the absence or dysfunction of these hydrolytic enzymes. Options B and C describe other possible lysosomal dysfunctions but are not the defining feature of LSDs. Option D is incorrect because the enzyme deficiency (Option A) is the hallmark of these disorders.