Multiple choice

Which of the following combinations of ‘restriction enzyme: bacterial source-recognition sequence’ is not correctly matched?

  1. EcoR-I: Escherichia coli- 5’ GAATTC; 3’ CTTAAG

  2. EcoR-II: Escherichia coli- 5’ GATATC; 3’ CTATAG

  3. BamH-I: Bacillus amyloliquefaciens- 5’ GGATCC; 3’ CCTAGG

  4. Hind-III: Haemophilus influenzae- 5’ AAGCTT; 3’ TTCGAA

  5. Taq-I: Thermus aquaties- 5’ TCGA; 3’ AGCT
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The restriction enzyme for Escherichia coli is EcoR-II with recognition sequence of 5’ CCWGC; 3’ GCWCC. The restriction enzyme for Escherichia coli is EcoR-V with recognition sequence of 5’ GATATC; 3’ CTATAG.

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