Multiple choice

A researcher creates an artificial vector. It has an ori region containing the sequence GAATTC. It also has a lacZ marker gene containing the sequence HindIII enzyme. Apart from these, there are restriction sites for EcoRII, BamHI and TaqI away from the marker gene. Now, he wants to cut out a target gene, which has restriction sites for the following restriction endonucleases. Which restriction enzyme should he use?

  1. EcoRI

  2. BamHI

  3. HindIII

  4. TaqI

  5. EcoRII
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

It’s the most appropriate choice. Its restriction site is present inside a marker gene and hence, the researcher will be able to screen for recombinants later.

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