The pyruvic acid transported into the mitochondrial matrix is not directly used in the Krebs cycle. First it loses a molecule of CO2 as NAD+ is reduced to NADH. The remaining molecules, called acetyl group, attaches to coenzyme to form acetyl coenzyme A. The conversion of pyruvic acid to acetyl CoA links glycolysis and the Krebs cycle. Pyruvic acid is the final product of glycolysis and acetyl CoA is the compound that enters the Krebs cycle.