Multiple choice

Gene targeting is a technique utilising homologous recombination between an engineered exogenous DNA fragment and the genome of an individual's embryonic stem (ES) cells. Which of the following regarding this technique is INCORRECT?

  1. It uses homologous recombination to change an endogenous gene.

  2. The frequency of gene targeting can be significantly enhanced by using endonucleases.

  3. The targeting construct made out of DNA is generated in bacteria.

  4. Gene targeting is a permanent method.

  5. It requires the creation of a specific vector.
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Gene targeting can be permanent or conditional. Conditions can be a specific time during development / life of the organism or limitation to a specific tissue.

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