Multiple choice

Gene targeting is a well known genetic technique. Which among the following statements regarding this technique is incorrect?

  1. Gene targeting is a permanent method.

  2. It requires the creation of a specific vector.

  3. The targeting construct made out of DNA is generated in bacteria.

  4. The frequency of gene targeting can be significantly enhanced by using endonucleases.

  5. It uses homologous recombination to change an endogenous gene.

Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Gene targeting can be permanent or conditional. Conditions can be a specific time during development or life of the organism or limitation to a specific tissue.