Multiple choice

The feature that best refers to the melting temperature of DNA is

  1. the temperature at which the entire DNA is denatured

  2. the length of DNA

  3. the proportion of G-C content of DNA

  4. the proportion of the purine content of DNA

  5. none of these
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

DNA melting temperature (Tm) is primarily determined by GC content because G-C pairs form three hydrogen bonds versus two for A-T pairs. Higher GC content means higher Tm due to stronger bonding.

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