Multiple choice

Which of the following processes is carried out during the preparation of chimeric DNA?

  1. Specific gene is isolated and amplified.

  2. Plasmids are replicated independently from bacterial DNA.

  3. Restriction endonuclease and human DNA are incubated together for annealing.

  4. Gene of interest & plasmid are cut with two different restriction enzymes.

  5. Restriction enzymes help in joining two DNA molecules.
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Creating chimeric DNA (recombinant DNA) begins with isolating and amplifying the specific gene of interest - you need sufficient copies of the target gene before combining it with a vector. Options B, D, and E describe other steps in recombinant DNA technology, while C incorrectly describes annealing without specifying the correct components.

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