Multiple choice

For creating a recombinant DNA molecule, which enzyme should be used to cut open the target gene and the carrier plasmid?

  1. DNAse for target gene and restriction endonuclease for plasmid

  2. DNAse for plasmid and restriction endonuclease for target gene

  3. DNAse for cutting both target gene and plasmid

  4. Restriction endonuclease for cutting both target gene and plasmid

  5. Restriction exonuclease for cutting both target gene and plasmid
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Cutting both the target gene and the carrier plasmid will create complementary sticky ends required for fitting the target gene into the carrier plasmid.

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