Multiple choice Structure separating atria of the heart from the ventricles is Coronary sulcus Interatrial groove Anterior interventricular sulcus Posterior longitudinal sulcus Reveal answer Fill a bubble to check yourself A Correct answer Explanation Atria of the heart are separated from the ventricles by the coronary sulcus, this contains the trunks of the nutrient vessels of the heart, and is deficient in front, where it is crossed by the root of the pulmonary artery.