Multiple choice Which of the following is the partition that separates the chambers of the heart? Pericardium Septum Fissure None of these Reveal answer Fill a bubble to check yourself B Correct answer Explanation The septum is the thick muscular wall that separates the left and right sides of the heart. This separation ensures that oxygenated and deoxygenated blood do not mix. The pericardium is the protective sac around the heart, not an internal partition.