During somatic hybridisation, which of the following enzymes are needed to obtain naked protoplasts?
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Cellulase and protease
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Cellulase and amylase
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Cellulase and pectinase
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Cellulase and lipase
C
Correct answer
Explanation
Plant cell walls contain cellulose and pectin as structural components. To obtain naked protoplasts during somatic hybridization, both cellulase (breaks down cellulose) and pectinase (breaks down pectin) are needed to digest the cell wall completely. Amylase (starch), protease (proteins), and lipase (lipids) are not relevant for cell wall digestion.