Multiple choice

Roll tube technique is used to obtain pure cultures of which of the following?

  1. Stringent anaerobes

  2. Facultative anaerobes

  3. Mycobacterium tuberculosis

  4. Mycoplasma
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A Correct answer
Explanation

Roll tube technique is used for stringent anaerobes. It provides an ideal condition for the cultivation of stringent anaerobes (redox potential below - 300 mV) which can be only achieved by the supplementation of media with reducing agents. This method consists of a roller tube in which the organism to be cultivated is immobilised on the side of the tube by a film of serum or other media.

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