Multiple choice

Helmstetter-Cummings technique is used

  1. to obtain pure culture

  2. for bacterial enumeration

  3. to obtain synchronous culture

  4. in plating the bacteria
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C Correct answer
Explanation

Helmstetter-Cummings technique is used to obtain synchronous culture. In the Helmstetter-Cummings technique, a bacterial culture is filtered through a membrane. Most bacteria pass through, but some remain bound to the membrane. The membrane is subjected to reverse flow of liquid medium. The cells in the effluent stream have predominantly the characteristics of newborn cells. Hence, they are synchronous.

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