Multiple choice

For which of the following is difference cloning useful?

  1. To isolate sequence of DNA that is expressed in specific tissue types.

  2. To detect the DNA sequence of a mutated gene.

  3. To detect biological activity of expressed polypeptide or protein.

  4. To isolate gene expressing antigenic peptides.

  5. To isolate specific DNA sequence from uncloned DNA fragments.

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A Correct answer
Explanation

Difference cloning is used to isolate sequences that are expressed in specific cell types.