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Molecular Biology and Life Sciences

CSIR UGC NET Life Sciences - covering molecular biology techniques, immunology, and developmental biology concepts

5 Questions Published
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Questions

Question 1 Multiple Choice (Single Answer)

What will be useful for the cloning of gene fragments having molecular weight above 300kb?

  1. Plasmid
  2. Phage
  3. Cosmid
  4. Bacterial Artificial chromosome
  5. Yeast artificial Chromosome
Question 2 Multiple Choice (Single Answer)

Which of the following methods is useful to separate DNA fragments of larger molecular mass?

  1. SDS-PAGE
  2. Agarose gel elctrophoresis
  3. Pulse field Gel electrophoresis
  4. 2D-Gel electrophoresis
  5. UV- Spectroscopy
Question 3 Multiple Choice (Single Answer)

A thermostable proof reading enzyme used for PCR reaction is _______.

  1. Taq polymerase
  2. DNA polymerase I
  3. Endonuclease
  4. Reverse transcriptase
  5. Pfu polymerase
Question 4 Multiple Choice (Single Answer)

Which of the following is not a property of λ phage vector?

  1. Use in cDNA library construction.
  2. Contain E.coli lacZ gene Driven by lac promoter.
  3. It can manipulate invitro.
  4. Both 2 and 3.
  5. only 3.
Question 5 Multiple Choice (Single Answer)

For which of the following is difference cloning useful?

  1. To isolate sequence of DNA that is expressed in specific tissue types.
  2. To detect the DNA sequence of a mutated gene.
  3. To detect biological activity of expressed polypeptide or protein.
  4. To isolate gene expressing antigenic peptides.
  5. To isolate specific DNA sequence from uncloned DNA fragments.
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