Multiple choice

During protein synthesis in eukaryotes, when does the tRNA-met bind to 5’ mRNA?

  1. Before binding of the small ribosomal subunit.

  2. After binding of the small ribosomal subunit.

  3. Before binding of the large ribosomal subunit.

  4. After binding of the large ribosomal subunit.

  5. Before binding of both the ribosomal subunits.
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A Correct answer
Explanation

The initiator tRNA (tRNAimet) binds to the 5’ end of mRNA before binding of the small (40S) ribosomal subunit. The large ribosomal subunit in eukaryotes is 60S subunit, which binds to 5’mRNA after initiator tRNA, the 40S subunit and initiation factors bind to mRNA.

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