Bio-agents are organic agents used in fertilisers. For example, culture of blue-green algae. Cultures of neem leaves or turmeric are used in grain storage as bio–pesticides because grains, during storage, cannot be sprayed with pesticides. So, during that period, cultures of neem leaves are placed with them. Culture of earthworms, used in making compost (rich in organic matter and nutrients), hastens the process of decomposition of waste. Culture of mushrooms cannot be used as mushrooms themselves are capable of extracting the nutrients from fertilisers.