Multiple choice How many moles of ATP are used when one mole of nitrogen is fixed? 2 16 8 1 Reveal answer Fill a bubble to check yourself B Correct answer Explanation 16 moles of ATPs are consumed when 1 mole of nitrogen is fixed. The reaction is as follows: N2 + 8H+ + 8e- + 16 ATP = 2NH3 + H2 + 16ADP + 16 Pi