Multiple choice

Which one of the following makes use of RNA as a template to synthesise DNA?

  1. DNA dependent RNA polymerase

  2. DNA polymerase

  3. Reverse transcriptase

  4. RNA polymerase.
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C Correct answer
Explanation

 The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptases (RTs) use an RNA template and a short primer complementary to the 3' end of the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR).

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