Multiple choice

Isoelectric focussing technique that is used to separate proteins works on the principle of

  1. electrophoretic separation based on relative content of acidic and basic residues

  2. mass of the protein molecules

  3. number of amino acids containing sulphur

  4. coagulation capacity of the protein molecules

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A Correct answer
Explanation

Isoelectric focussing (IEF) is an electrophoretic method that separates proteins according to their isoeletric points(pI). proteins carry both positive and negative charges and their net charge depends highly on the pH of their surroundings. the pI is the specific pH at which the net charge on a protein molecule is zero. Thus, Isoelectric focussing technique used to separate proteins works on the principle of electrophoretic separation based on relative content of acidic and basic residues.

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