Multiple choice

The purpose of adding phytohemagglutinin to peripheral blood cultures for chromosomal analysis is

  1. swelling of cells to permit chromosome visualization

  2. collection of large number of cells at metaphase

  3. chromosome condensation

  4. stimulation of lymphocyte cell division

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Phytohemagglutinin stimulates the division of T-cells. A mitotic arresting drug such as colchicine is used to collect dividing cells at metaphase and hypotonic treatment swells the cells to permit chromosomes to be visualized.