Tag: denaturation of protein

Questions Related to denaturation of protein

The diameter of a double helix is:

  1. 20 A$^{0}$

  2. 0.02 A$^{0}$

  3. 0.2 A$^{0}$

  4. none


Correct Option: A
Explanation:

Through X-ray crystallographic methods, it was determined that the diameter of the double helix is around 2 nm. Also, the structure repeats itself after every 3.4 nm.

The separation of two DNA stands by heating is called

  1. melting temperature

  2. annealing

  3. denaturation

  4. boiling


Correct Option: C
Explanation:

The separation of two DNA stands by heating is called denaturation. The intermolecular hydrogen bonds and disulfde linkages break.

Globular Proteins are present in:

  1. Blood

  2. Eggs

  3. Milk

  4. All the above


Correct Option: D
Explanation:

Globular Proteins have three dimensional folded structure. These are cross linked proteins, soluble in water.
Globular Proteins are present in blood, eggs and milk.

Which of the following statements are true about (X) and (Y)?
X)  Globular Protein               Y)  Fibrous Protein
a.These are linear condensation polymers.
b. Insoluble in water.
c. These proteins have three dimensional folded structure.
d. These are cross linked protein.
e. Soluble in water
f. These are held together by intermolecular hydrogen bond.

  1. X = a, b, c; Y = d, e, f

  2. X = c, d, e; Y = a, b, f

  3. X = b, c, a; Y = c, b, f

  4. X = a, e, f; Y = b, c, f


Correct Option: B
Explanation:

Globular Proteins have three dimensional folded structure.
These are cross linked protein.
They are Soluble in water.
Fibrous Proteins are linear condensation polymers.
They are Insoluble in water.
These are held together by intermolecular hydrogen bond.

The secondary structure of a protein refers to:


  1. $\alpha$-helical back bone

  2. hydrophobic interations

  3. Sequence of $\alpha$-amino acids

  4. fixed configuration of the polypeptide backbone


Correct Option: A
Explanation:

The secondary structure of a protein refers to either of $\alpha$-helical back bone or $\beta$-sheet.

Which of the following is a globular protein?

  1. Collagen

  2. Myoglobin or Haemoglobin

  3. Myosin

  4. Fibroin


Correct Option: B
Explanation:

Myoglobin or haemoglobin is a globular protein.


Collagen, myosin and fibroin are fibrous protein.

Option B is correct.

Globular proteins are present in:

  1. Eggs

  2. Muscles

  3. Keratin

  4. Blood


Correct Option: A,D
Explanation:

 Globular proteins are present in egg albumin, haemoglobin, myoglobin, insulin, serum globulins in blood, and many enzymes.

The main structural feature of proteins is_________.

  1. Ether linkage

  2. Ester linkage

  3. Peptide linkage

  4. All the three above


Correct Option: C
Explanation:

Proteins are linear polymers formed by linking the -carboxyl group of one amino acid to the -amino group of another amino acid with a peptide bond.

Which structural feature distinguishes proline from other natural $\alpha$-amino acids :

  1. It is optically inactive

  2. It contains aromatic group

  3. It is dicarboxylic acid

  4. It has a secondary amine

  5. It contains two amino groups


Correct Option: D
Explanation:

Proline is the only proteinogenic amino acid whose side-group links to the -amino group and, thus, is also the only proteinogenic amino acid containing a secondary amine at this position.

The sequence in which amino acids are linked to one another in a protein molecule is called its:

  1. Primary structure

  2. Secondary structure

  3. Tertiary structure

  4. Quanternary structure


Correct Option: A
Explanation:

Proteins may have one or more polypeptide chains. Each polypeptide in a protein has amino acids linked with each other in a specific sequence and it is this sequence of amino acids that is said to be the primary structure of that protein.