Tag: manipulation of dna

Quizzes (0) Questions (50)

Questions Related to manipulation of dna

Multiple choice botany biotechnology: principles and processes manipulation of dna electrophoresis isolation and separation of dna

A technique utilized by a group of biologists to isolate an antifreeze gene found in cold water species of fish and introduce it into the genome of a different organism is called as 

  1. Cloning

  2. Artificial selection

  3. Selective breeding

  4. Genetic engineering

  5. Sexual recombination
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation
Genetic engineering is the branch of science which deals with the modification of genes. The desired genes is isolated from the host like the gene for antifreeze protein and are inserted into the vectors. The vectors carries the foreign gene into the desired organism like the fish and then the gene gets introduced into the genome of fish, resulting in the production of the protein.
Thus, the correct answer is option 'Genetic engineering'.
Multiple choice bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would you find the smallest segments of DNA?

  1. Near the positive electrode, farthest away from the wells

  2. Near the negative electrode, close to the wells

  3. Near the negative electrode, farthest away from the wells

  4. Near the middle, they tend to slow down after the first few minutes
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Since DNA is itself negatively charged, it would move towards the positive electrode. In gel electrophoresis, DNA fragments are separated on the basis of charge and masses. Thus, smaller the DNA fragment farther it moves from the well.

So, the correct option is A.

Multiple choice bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

Gel electrophoresis is used for

  1. Construction of recombinant DNA by joining with cloning vectors

  2. Isolation of DNA molecules

  3. Cutting of DNA into fragments

  4. Separation of DNA fragments according to their size
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are separated according to their size. 

So, the correct option is 'Option D' .

Multiple choice bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

Select the correct option to fill up the blanks.


(i) _____ is a natural polymer extracted from ______.

(ii) The DNA fragments purified by gel electrophoresis are used in constructing _____ by joining them with ______.
(iii) The ligation of alien DNA is carried out at a _____ present in one of the two ____ in a plasmid vector.
(iv) _____ enzyme remains active during the high temperature-induced denaturation of ds DNA. DNA fragments are resolved according to their ______
(v) through _____ in agarose gel electrophoresis.

  1. (i) Agarose, seaweeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase, size(v) sieving effect.

  2. (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect

  3. (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii A-DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect

  4. (i) Size, sieving effect (ii) agarose, sea weeds (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Agarose is a natural polymer extracted from sea weeds. The DNA fragments purified by gel electrophoresis are used in constructing recombinant DNA by joining them with the cloning vector. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes in a plasmid vector. Taq polymerase enzyme remains active during the high temperature-induced denaturation of dsDNA.  DNA fragments are resolved according to their size through a sieving effect in agarose gel electrophoresis.

So, the correct option is 'Option A'.