Blood does not contain
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Calcium
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Prothrombin
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Fibrinogen
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Elastin
Blood clotting factors are needed for blood to clot (coagulation). Prothrombin is one of the clotting factors made by the liver. During normal blood coagulation, a coagulation cascade activates prothrombin by converting it into the serine protease thrombin. Thrombin then converts the soluble fibrinogen into insoluble fibrin strands. These strands are then cross-linked by factor XIII to form a blood clot. As soon as blood from a wound is exposed to the air, the platelets disintegrate and react with fibrinogen to create fibrin: a mass of tiny threads. This triggers a whole series of reactions that rely on adequate levels of calcium and vitamin K to work. Calcium is also an important part of the blood clotting process. Elastin is a highly elastic protein in connective tissue and allows many tissues in the body to resume their shape after stretching or contracting. It is not present in the blood.