Tag: world of microbes

Questions Related to world of microbes

The smear of herpes virus is stained blue with

  1. 1% methylene blue

  2. 1% alcian blue

  3. 1% toluidine blue

  4. 1% coomassie blue


Correct Option: C
Explanation:
  • Fluid or tissue scrap taken from the lesions and sores that appear in early stages of disease are used to diagnose if the patient is infected with viral infection or not.
  • The stains used are such that it may stain the acidic genetic material present in the rapidly dividing cells of the virus or bacteria
  • Since the fluid that is taken from the early lesions that appear on the skin in the early stage of the disease have syncytial condition ie more amount of genetic material is present therefore the staining will take place properly.
  • 1% toluidine blue is basic dye that will stain the nucleic acid blue.
  • Methylene blue is used to observe microscopic life under the microscope. It usually stains the outer wall or membrane of the organism.
  • Alcian blue is used to stain acidic polysaccharide in muscles and tissue in histological studies.
  • Coomassie blue is used for studying proteins as it stains the protein band blue color so that it can be observed as blue bands with a clear background as it does not stain any other thing in cell.
  • Therefore the option C is correct as toluidine is the only dye her which will stain the acidic genetic material in the dividing cells of the virus.
  • So, the correct answer is '1% toluidine blue'

To avoid transmission of STDs we should
(i) Avoid sex with multiple partners
(ii) Always have unprotected sex
(iii) Use condoms during coitus
(iv) Avoid sex with unknown partners
(v) Avoid sharing of needles

  1. (i), (ii), (iii), (iv) and (v)

  2. (i), (iii), (iv) and (v)

  3. (i), (ii) and (iii)

  4. (i), (ii) and (iv)


Correct Option: B
Explanation:

STD's are the sexually transmitted disease that can be transferred from one organism to another by the exchange of body fluids.

For the prevention of STD one should avoid sex with multiple partners, always have protected sex and use condoms.
One should avoid having sex with unknown partners. 
Avoid sharing used needles as it is seen in the case of many hospitals and drug addicts.
So, the correct option is '(i), (iii), (iv) and (v)'