Biology · Environmental Studies
Microbiology and Nitrogen Cycle
950 Questions
This hub focuses on microbiology and the nitrogen cycle, covering essential concepts about bacteria and soil microbes. Topics include nitrogen fixation, bacterial structures, and soil microbial processes. These concepts are frequently tested in general science sections of various competitive exams.
Nitrogen fixing bacteriaGram-positive bacteriaBacterial flagellaDenitrifying bacteriaAmmonia conversion processBacterial resting spores
Microbiology and Nitrogen Cycle Questions
Match organisms in Group I with shapes in Group II and flagellar arrangements in Group III and select the correct answer.
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| Group I |
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Group II |
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Group III |
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| P. Escherichia coli |
i. Spherical |
1. Peritrichous |
| Q. Staphylococcus aureus |
ii. Comma-shaped |
2. Atrichous |
| R. Vibrio cholerae |
iii. Rod-shaped |
3. Monotrichous |
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P - ii - 3, Q - iii - 1, R - i - 2
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P - iii - 1, Q - i - 2, R - ii -3
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P - i - 2, Q - ii - 3, R - iii - 1
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P - ii - 2, Q - iii - 1, R - i - 3
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P - i - 2, Q - ii - 1, R - iii - 3
B
Correct answer
Explanation
Escherichia coli is a Gram-negative, facultatively anaerobic, rod-shaped bacterium. The flagella in E. coli have a peritrichous arrangement.
Staphylococcus aureus is an atrichous, Gram-positive, sphere-shaped bacteria that form clusters.
Vibrio cholerae is a monotrichous, Gram-negative, comma-shaped bacterium.
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Aulosira
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Anabaea
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Nostoc
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All of these
D
Correct answer
Explanation
Aulosira, Anabaena, and Nostoc are all blue-green algae (cyanobacteria) capable of atmospheric nitrogen fixation. They possess nitrogenase enzymes for this purpose. The question correctly identifies that all these genera have nitrogen-fixing capability. Note: 'Anabaea' appears to be a typo for 'Anabaena'.
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Rhodospirillum
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Rhodopila
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Rhodobacter
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All of these
D
Correct answer
Explanation
Rhodospirillum, Rhodopila, and Rhodobacter are all photosynthetic bacteria capable of anoxygenic photosynthesis using bacteriochlorophyll. These purple non-sulfur and purple sulfur bacteria use organic compounds or sulfur compounds as electron donors instead of water.
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air
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water
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soil
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human intestine
D
Correct answer
Explanation
Escherichia coli (E. coli) is a bacterium that naturally inhabits the human intestinal tract as part of the normal gut microbiota. While it can be found in water and soil due to fecal contamination, its primary habitat is the human intestine where it aids in digestion and vitamin K production.
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Catalase
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Arginase
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Urease
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Nitrogenase
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Hydrogenase
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only a
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b and c
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only c
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a and b
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only b
A
Correct answer
Explanation
This is the correct option, as rhizobium bacteria do not live in the stem of legumes such as gram, peas and other pulses. They live in the roots of legumes and provide them with nitrogen. In return, the plants provide food and shelter to the bacteria.
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Rhizobium
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Nitrosomonas
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Nitrobactor
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Nitrococcus
A
Correct answer
Explanation
Rhizobium is the most well-known symbiotic nitrogen-fixing bacterium, forming mutualistic associations with legume plants in root nodules. The bacteria convert atmospheric nitrogen into ammonia that the plant can use, while receiving carbohydrates from the plant. Nitrosomonas and Nitrobacter are involved in nitrification, not nitrogen fixation.
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clostridium
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acetobactor
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azotobactor
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nostoc
A
Correct answer
Explanation
Clostridium is a genus of anaerobic, free-living bacteria that includes species capable of fixing atmospheric nitrogen. Unlike Rhizobium (which forms symbiotic relationships with legumes) or Azotobacter (which is aerobic), Clostridium species can fix nitrogen independently in anaerobic conditions. These bacteria play important roles in soil nitrogen cycling, particularly in waterlogged or oxygen-poor environments.
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Coccus
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Vibrio
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Bacillus
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Spirillum
B
Correct answer
Explanation
Vibrio is the coma-shaped (curved) bacterium. Coccus is spherical, Bacillus is rod-shaped, and Spirillum is spiral-shaped. The distinctive comma shape is characteristic of Vibrio cholerae.
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Legumes
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Artemesia
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Rhizobium
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Azolla
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Anabena
B
Correct answer
Explanation
It is a large, diverse genus of plants with between 200 and 400 species belonging to the daisy family Asteraceae. Asteraceae family is not involved in nitrogen fixation.
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Spirillum
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Bacillus
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Dialister
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Both (1) and (2)
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None of these
C
Correct answer
Explanation
Dialister pneumosintes is the smallest bacterium (0.15—0.3 n long).
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Cyanobacteria
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Myxobacteria
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Mycobacteria
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Archaebacteria
D
Correct answer
Explanation
Biogas plants rely on methanogenic archaebacteria (archaea) to convert organic matter into methane-rich biogas through anaerobic digestion. Cyanobacteria are photosynthetic, myxobacteria are soil bacteria, and mycobacteria include pathogens like TB - none are involved in biogas production.
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Parasitism
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Symbiosis
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Commensalism
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Space parasitism
B
Correct answer
Explanation
The rhizosphere is the narrow region of soil directly influenced by root secretions and associated soil microorganisms. These microorganisms have symbiotic relationships with plants - they help in nutrient absorption, nitrogen fixation, and plant growth promotion in exchange for root exudates as food.
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Photoautotrophic
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Chemoautotrophic
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Heterotrophic
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Parasitic
B
Correct answer
Explanation
These organisms can utilise energy released during the chemical reaction occurring in them. Sulphur and iron bacteria are chemoautotrophic.
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a and b
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a, b and c
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a, b, c and d
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a, b and d
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b, c and d
B
Correct answer
Explanation
This is the correct asnwer as soil microorganisms and associated plant roots, lightning and cosmic radiation and some marine animals can fix nitrogen from the atmosphere.