Biology
DNA and Genetics
1,095 Questions
DNA and genetics questions explore molecular biology, DNA replication, and genetic engineering techniques. These topics are essential for various competitive exams requiring a strong foundation in life sciences. Practice these questions to understand complex biological processes thoroughly.
DNA replication mechanismsGenetic engineering techniquesGene editing toolsPolymerase chain reactionDNA sequencing methods
DNA and Genetics Questions
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Lac operon
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Trp operon
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Lac repressor
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Gal operon
B
Correct answer
Explanation
This operon is found in many bacteria, but was first noticed in Escherichia coli. It is regulated so that when tryptophan is present in the environment, it is not used.
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Structural gene
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Operator gene
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Promoter gene
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Regulator gene
D
Correct answer
Explanation
This gene regulates the operator gene with the help of a chemical substance known as inducer present in the cytoplasm. It is not certainly present close to the operator gene which it controls.
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plasmids used in DNA probe
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plasmids helpful in bioremediation
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plasmids used to produce disease-resistant plants
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plasmids to promote conjugation
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none of the above
B
Correct answer
Explanation
OCT, XAM and CAM are plasmids seen in Pseudomonas putida as anti-pollutants.
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restriction endonuclease
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DNA-ligase
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DNA-polymerase
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RNA-polymerases
A
Correct answer
Explanation
The enzyme used to cut the DNA molecule at specific site is restriction endonuclease, also known as molecular scissors.
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stitching of genes
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isolation of genes
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sequencing of DNA
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insertion of genomic clones into a vector
D
Correct answer
Explanation
Shotgun technique is employed in insertion of genomic clones into a vector.
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create terminate codon
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manufacture antibiotics
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differentiate different biotypes of gall midge
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obtain soil microorganisms with the help of specific enzyme reductase
C
Correct answer
Explanation
Polymerase chain reaction (PCR) based markers have been developed to differentiate different biotypes of gall midge.
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point mutation
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translocations
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deletion
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gene amplification
D
Correct answer
Explanation
HER-2/neu activation in breast cancer is based on gene amplification and is an important prognostic indicator.
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Nif - plasmid
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R - plasmid
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Ti - plasmid
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F - plasmid
C
Correct answer
Explanation
Ti-plasmid induces tumor formation in plants.
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mutual activity of operator and promoter genes
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central biological dogma
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reverse transcription
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transduction in bacteria
C
Correct answer
Explanation
Reverse transcription is the formation of DNA from RNA by enzyme reverse transcriptase. This phenomenon was discovered by ‘Tamin and Baltimone’ and thus, known as ‘Teminism’.
B
Correct answer
Explanation
D regions are only found in the heavy chain; the light chain gene loci lack D segments.
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DNA pol I
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DNA pol II
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DNA pol III
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DNA pol IV
C
Correct answer
Explanation
DNA pol III is the first polymerase to participate in DNA replication.
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DNA pol III
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DNA pol II
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DNA pol I
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DNA ligase
C
Correct answer
Explanation
DNA pol I fills the necessary nucleotides at gaps formed by excision of RNA primer between the okazaki fragments.
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Polymerisation
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Proofreading
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Joining of okazaki fragments
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Excision of RNA primer
B
Correct answer
Explanation
The proofreading function of DNA polymerase I is carried out by a 3' to 5' exonuclease.
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Primase - Helicase - ssBP - Polymerase
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Primase - ssBP - Helicase - Polymerase
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Helicase - ssBP - Primase - Polymerase
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Helicase - Primase - ssBP - Polymerase
C
Correct answer
Explanation
DNA replication starts when helicase unzips DNA helix.............ssBP stabilises the unwound strands..............primase synthesises RNA primer...........and then, polymerase carries out the synthesis.
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RNA polymerase
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Helicase
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DNA polymerase
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Ligase
C
Correct answer
Explanation
DNA polymerase catalyses the elongation of the leading strand.