Methods in Microbiology (GATE)

Fungi are chemoorganoheterotrophs and use organic compounds as a source of carbon, electrons and energy.

Rhodobacter is a photosynthetic bacterium. It uses photon energy for making food, organic oxidising donor source and organic carbon source. Thus, it is a photoorganoheterotroph.

Calcium is a component of endospores and provides heat resistance to them.

Potassium is required for the activity of enzymes involved in protein synthesis. Thus, protein synthesis in bacteria would suffer due to the deficiency of potassium.

An organic nutrient provider should at least provide C and H.

Albert staining is used to stain the volutin or metachromatic granules of C. diphtheria. On Albert staining, the metachromatic granules appear blue in color.

Oceanithermus profundus follows chemolithoheterotrophic mode of nutrition. It is a moderately thermophilic, organotrophic, microaerophilic and facultatively chemolithotrophic bacterium isolated from a deep-sea hydrothermal vent site at 13-N in the East Pacific Rise.

Biotin is essential for bio-synthetic reactions that require CO₂ fixation.

Samples are passed through propylene oxide in the process of embedding. After treating with propylene oxide, samples are infiltrated with an epoxy resin and then embedded directly in water-miscible acrylic resin.

This is true. In DIC, image is similar to that obtained by phase contrast microscopy but without the bright diffraction halo.

Chroococcales is a mixotroph. It can switch between different metabolic modes like photoautotrophy, photoheterotrophy and chemoheterotrophy.

Ammonia-oxidising bacteria are chemolithotrophic bacteria that get carbon for biosynthesis from carbon dioxide.

Enterococcus faecalis is an aerotolerant organism as it only contains peroxidase.

Green non-sulphur bacteria are photoheterotrophs. They use light for energy and use organic compounds to satisfy their carbon needs.

The above mentioned medium would act as a defined enrichment medium for the growth of Thiobacillus thiooxidans. It is chemically defined and it contains elemental sulphur as carbon source, enriching it for the growth of Thiobacillus thiooxidans.