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Recombinant DNA Technique (Level : Hard)

Recombinant DNA technology covering restriction enzymes, plasmids, transformation, and genetic engineering techniques

15 Questions Published
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Questions

Question 1 Multiple Choice (Single Answer)

Arrange the following in the sequence of their usage in recombination DNA technique.

  1. CaCl2
  2. Ligase
  3. EDTA
  4. Restriction endonuclease
  1. 1, 2, 3, 4
  2. 4, 1, 3, 2
  3. 3, 1, 2, 4
  4. 4, 3, 2, 1
Question 2 Multiple Choice (Single Answer)

Restriction endonucleases are also known as

  1. molecular glue
  2. molecular scissors
  3. molecular markers
  4. reverse transcriptase
Question 3 Multiple Choice (Single Answer)

Which of the following is a palindromic sequence?

  1. 5'- T T G A G T -3'3'- A A C T C A -5'
  2. 5'- G A A T T C -3'3'- C T T A A G -5'
  3. 5'- A A C C A G -3'3'- T T G G T C -5'
  4. 5'- A C T C T G -3'3'- T G A G A C -5'
Question 4 Multiple Choice (Single Answer)

Plasmids are autonomously replicating minichromosomes found in

  1. bacteriophages
  2. E. coli
  3. amoeba
  4. paramecium
Question 5 Multiple Choice (Single Answer)

Gel electrophoresis is used to separate

  1. RNA
  2. DNA
  3. protein
  4. All of the above
Question 6 Multiple Choice (Single Answer)

According to EFB (European Federation of Biotechnology), biotechnology is the integration of natural science and

  1. organisms
  2. cells
  3. molecular analogs
  4. All of the above
Question 7 Multiple Choice (Single Answer)

Which of the following methods uses a gene gun for genetic engineering?

  1. Vector method
  2. PCR technique
  3. Bolistic method
  4. Plasmid method
Question 8 Multiple Choice (Single Answer)

In recombinant DNA technology, the purified DNA is precipitated by

  1. distilled water
  2. chilled ethanol
  3. ethanol at 36°C
  4. 70% ethanol
Question 9 Multiple Choice (Single Answer)

Who is the father of genetic engineering?

  1. Herbert Boyer
  2. Paul Berg
  3. Rudolf Jaenisch
  4. Jack Williamson
Question 10 Multiple Choice (Single Answer)

What is the full form of GMO?

  1. Genetically manipulated organism
  2. Genetically modified organism
  3. Genetically maneuvered organism
  4. Generating mutated organism
Question 11 Multiple Choice (Single Answer)

The first genetically altered primate was

  1. Dolly
  2. Herman
  3. Andi
  4. Polly
Question 12 Multiple Choice (Single Answer)

Which of the following techniques uses homologous recombination to target desired changes to a specific endogenous gene?

  1. Genome editing
  2. Gene targeting
  3. Gene trapping
  4. Gene splicing
Question 13 Multiple Choice (Single Answer)

Which of the following is a class of restriction enzymes?

  1. Nucleolase
  2. Exonuclease
  3. Endonuclease
  4. Transcriptase
Question 14 Multiple Choice (Single Answer)

Which of the following sentences about plasmids is untrue?

  1. Plasmid is always a double-stranded DNA molecule.
  2. Plasmid is always circular.
  3. Plasmid is coated with histone protein.
  4. Plasmid does not carry any vital gene necessary for the cell.
Question 15 Multiple Choice (Single Answer)

What is the result of calcium treatment of bacteria?

  1. Bacteria becomes resistant to ampicillin.
  2. Bacteria becomes more permeable to rDNA.
  3. Bacteria gets stained.
  4. Bacterial DNA gets precipitated.
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