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DNA Finger Printing (Level : Easy)

DNA fingerprinting techniques, including VNTRs, satellites, RFLP analysis, and applications in forensics and parentage testing.

15 Questions Published
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Questions

Question 1 Multiple Choice (Single Answer)

Mini-satellites are

  1. 9bp to 80bp sized short sequences of DNA
  2. short coding repetitive regions on the eukaryotic genome
  3. short non-coding repetitive sequences present on chromosomes
  4. region of chromosome after secondary constriction
Question 2 Multiple Choice (Single Answer)

Who discovered DNA fingerprinting?

  1. Ian Wilmut
  2. Paul Berg
  3. Sir Alec Jeffreys
  4. Herbert Boyer
Question 3 Multiple Choice (Single Answer)

Which of the following techniques is used for the separation of DNA fragments in DNA fingerprinting?

  1. Southern blotting
  2. Auto-radiography
  3. Electrophoresis
  4. Density gradient centrifugation
Question 4 Multiple Choice (Single Answer)

Which of the following techniques is used in DNA fingerprinting?

  1. Southern blot
  2. Western blot
  3. Northern blot
  4. FACS
Question 5 Multiple Choice (Single Answer)

What is rif-lip technique used for?

  1. DNA profiling
  2. Genome mapping
  3. Determination of the risk of a disease
  4. All of the above
Question 6 Multiple Choice (Single Answer)

When DNA fragments with significantly different base compositions are separated and monitored by absorption spectra of UV-light, the highest peak observed is of

  1. genomic DNA
  2. satellite DNA
  3. proteins associated with DNA
  4. telomereic DNA
Question 7 Multiple Choice (Single Answer)

The DNA fingerprint of a male child is similar to

  1. his father
  2. his mother
  3. 50% to his father and 50% to his mother
  4. no similarity to any of the parents
Question 8 Multiple Choice (Single Answer)

Which of the following has/have played a major role in DNA fingerprinting?

  1. Telomeres
  2. Satellites
  3. Centromeres
  4. Secondary constriction
Question 9 Multiple Choice (Single Answer)

Why is RFLP analysis done?

  1. To know base sequence of DNA fragment
  2. To detect change in the length of the restriction fragments
  3. To amplify smaller DNA segments
  4. To analyse chromosomes on basis of their shape to form a karyotype
Question 10 Multiple Choice (Single Answer)

Which probe is used for the identification of tandem repeats in DNA fingerprinting?

  1. TaqMan-probe
  2. MB probe
  3. VNTR-probe
  4. LNA prime time probe
Question 11 Multiple Choice (Single Answer)

Which of the following differences form(s) the basis of DNA fingerprinting?

  1. Difference in pattern of genes between individuals
  2. Difference in junk DNA pattern between individuals
  3. Difference in order of genes between individuals
  4. All of the above
Question 12 Multiple Choice (Single Answer)

Which of the following sequences of DNA fingerprinting procedure is correct?

  1. Restriction digestion - DNA isolation - Electrophoresis - Southern blotting - Probe hybridisation - Autoradiograph
  2. DNA isolation - Electrophoresis - Restriction digestion - Southern blotting - Probe hybridisation - Autoradiograph
  3. DNA isolation - Restriction digestion - Electrophoresis - Probe hybridisation - Southern blotting - Autoradiograph
  4. DNA isolation - Restriction digestion - Electrophoresis - Southern blotting - Probe hybridisation - Autoradiograph
Question 13 Multiple Choice (Single Answer)

DNA fingerprinting is applicable everywhere except

  1. to settle disputed parentage
  2. criminal identification and forensics
  3. personal identification
  4. amplification of VNTR region
Question 14 Multiple Choice (Single Answer)

A slight difference in the base-pair sequence of the common genes between individuals is known as

  1. dimorphism
  2. polymorphism
  3. mutation
  4. short tandem repeats
Question 15 Multiple Choice (Single Answer)

Phenotypic appearance of each individual is unique because

  1. entire DNA of all individuals is different
  2. repetitive sequences of each individual are unique
  3. telomeres of each individual are unique
  4. size of chromosomes of each individual is unique
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