Life Sciences (UGC / NET)
CSIR UGC NET Lifesciences Life Sciences
Questions
Exome analysis is useful in
- complete analysis of genes of an organism
- sequencing of intervening sequences
- getting information about protein coding sequences
- All of the above
- Only 2 and 3
Which of the following statements is/are true about isoelectric point of a protein?
- All proteins with isoelectric point below pH 7.0 will have negative charge and move towards positive electrode.
- All proteins with isoelectric point above pH 7.0 will have positive charge and move towards negative electrode.
- At isoelectric point, the net charge of protein depends on the nature of aminoacid present in the protein.
- All of the above
- Only 1 and 2
Which of the following statements is/are true about the generation of pH gradient in isoelectric focusing of protein?
- pH gradient is generated using ampholytes.
- Ampholytes have high buffering capacity at isoelectric point.
- Ampholytes are mixture of heteropolymers of oligoamino and oligo carboxylic acids.
- Only 1 and 2
- All of the above
Which of the following is/are thermostable DNA polymerase?
- Taq
- Vent
- Pfu
- None of these
- All of the above
Which of the following statements is/are about the intracellular ion concentration (Na, K, Ca) as compared to the concentration of that particular ion in the blood?
- Concentration of Na is higher inside the cell.
- Concentration of K is lower inside the cell.
- Concentration of Ca is higher inside the cell.
- Concentration of potassium is high inside the cell.
- Options 2 and 3 are correct.
The first stable compound of C4 cycle is
- oxaloacetic acid
- phospho enol pyruvate
- ribulose 1,5-diphosphate(RuDP)
- fructose 1,6 diphosphate
- phosphoglyceric acid
In biotechnology, dioxygenin is used for
- detection of fluid mosaic model of biological membranes
- detection of protein in the sample
- detecting antibodies
- detection of nucleic acids
- None of these
Which of the following statements is true about nissil bodies?
- It takes part in DNA replication.
- It is made up of rough endoplasmic reticulum (RER).
- Nissil bodies are involved in transcription.
- Nissil bodies are involved in proof reading while DNA replication.
- Nissil bodies are made up of golgi bodies.
Which of the following statements is true about enolpyruvylshikimate phosphate (EPSP) synthase?
- It converts phosphoenol pyruvate to pyruvate.
- Glyphosate induces EPSP synthase.
- Inhibition of EPSP synthase blocks aromatic acid synthesis.
- Underproduction of EPSP synthase is helpful in somatic embryogenesis.
- EPSP synthase is not required for plant cells.
Alkaptonuria is a condition in which
- oxidation of homogentisic acid takes place in the kidney
- urine becomes black while exposed to air
- patients lack enzymes to oxidise homogentisic acid
- Both 1 and 2 are correct.
- Both 2 and 3 are correct.
Which of the following statements is true about polytene chromosome?
- They are inert cellular objects seen in drosophilla.
- Banding pattern of polytene chromosome is constant in individuals of one species.
- Puffs seen in polytene chromosomes are the sites of gene expression.
- Giant chromosomes are not useful in studies of gene expression system.
- Formation of giant chromosomes happen in adult fly.
Which of the following statements is correct about transcription factors?
- Transcription factors help in unwinding DNA.
- Transcription factors help in recognising promoter sequence.
- Transcription factors recognise ori site.
- Transcription factors are involved in proof reading of newly synthesised RNA.
- None of these
In a bacterial gene, the sequence TTGACA is known as
- pribnow box
- consensus sequence
- conservative sequence
- ori site
- None of these
In development of a plant embryo, perisperm is
- integument
- primary endosperm nucellus
- part of nucellus persisting as a thin, peppery covering
- micropyle
- seed stalk
Which of the following sequences is true for prokaryotic transcription?
- Separation of DNA strands, joining of RNA polymerase, release of sigma factor, longation complex formation
- Separation of DNA strands, release of sigma factor, joining of RNA polymerase, elongation complex formation
- Joining of RNA polymerase, separation of DNA strands, release of sigma factor, longation complex formation
- Separation of DNA strands, joining of RNA polymerase, elongation complex formation, release of sigma factor
- Separation of DNA strands, elongation complex formation, joining of RNA polymerase, release of sigma factor