Biotechnology and Its Applications - 1
Covers genetic engineering techniques, molecular biology tools, and industrial applications of biotechnology
Questions
Match the biologists given in Column I with their specific fields of achievements given in Column II.
| Column I | Column II |
| A. Alec Jefferey | i. Cytogenetics and radiation genetics in plants |
| B. M. S. Swaminathan | ii. Function of DNA ligase |
| C. Eli Lily | iii. DNA finger printing technology |
| D. Mertz and Devis | iv. Preparation of humulin |
- A – ii, B – i, C – iv, D – iii
- A – iii, B – i, C – iv, D – ii
- A – iv, B – iii, C – ii, D – i
- A – ii, B – iv, C – i, D – iii
OCT, XAM and CAM are
- plasmids used in DNA probe
- plasmids helpful in bioremediation
- plasmids used to produce disease-resistant plants
- plasmids to promote conjugation
- none of the above
The process of preserving germ plasm in frozen state is called
- cryopreservation
- cold storage
- in situ preservation
- vernalisation
Colchicine brings about:
- chromosome aberrations
- doubling of chromosomes
- gene mutations
- quick replication
Hypervariables are useful in
- DNA fingerprinting
- genetic engineering
- cloning
- gene shearing
Yeast-two hybrid system is used to study
- yeast replication
- yeast protein synthesis
- protein-protein interaction
- transcription
Directions: The following question has four choices out of which ONLY ONE is correct.
What is antisense technology?
- RNA polymerase producing DNA
- A cell displaying a foreign antigen used for synthesis of antigens
- Production of somaclonal variants in tissue cultures
- When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
Industrial production of enzymes, vitamins, vaccines, biofertilisers, etc. is done by
- tissue culture
- cloning
- fusion techniques
- biotechnology
The first human hormone produced by recombinant DNA technology was
- STH
- thyroxin
- insulin
- None of these
A transgene can
- prevent the expression of a native gene
- modify an existing biosynthetic pathway
- produce a protein that itself produces a phenotype of interest
- All of the above
Electroporation in genetics is
- introduction of genetic material to a transgenic animal
- introduction of genetic material through viruses
- manipulation of cells by exposing them to strong electrical field
- injecting DNA into the egg through electrophoresis
Shotgun technique is employed in
- stitching of genes
- isolation of genes
- sequencing of DNA
- insertion of genomic clones into a vector
Useful genes are incorporated into a variety through genetic engineering method of crop improvement by means of
- plasmids
- microinjection
- co-cultivation
- All of the above
A radioisotope used to label proteins differently from nucleic acids is
- 14C
- 15N
- 32P
- 35S
Modern detergents contain enzyme preparations of
- thermophiles
- acidophiles
- alkaliphiles
- thermoacidophiles
PCR based markers have been developed to
- create terminate codon
- manufacture antibiotics
- differentiate different biotypes of gall midge
- obtain soil microorganisms with the help of specific enzyme reductase